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A study was carried out to compare the analytical performance of three different real-time PCR methods for the simultaneous detection of Ochratoxin A and citrinin, based on different TaqMan™ probes and TaqMan™ MGB™ probes, in cocoa beans. Triplicate samples of a commercial cocoa bean were analysed using three different methods with three different probes and their respective TaqMan™ probes. The order of sensitivity for both toxins, based on Cq values, using the TaqMan™ MGB™ Probe was 18.64 for Ochratoxin A and 11.71 for citrinin. The overall data obtained using different probes showed a good correlation with all three methods giving an average standard deviation of 0.32. The sensitivity of the three methods was all above 88.1%, the quantification factor was always lower than 1.5 and the precision of the three methods was all below 5.2%. The ability to detect target toxins in cocoa samples using the currently reported TaqMan™ MGB™ probes showed satisfactory results and could be applied to the field after further validation studies.Czarności

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A study was carried out to compare the analytical performance of three different real-time PCR methods for the simultaneous detection of Ochratoxin A and citrinin, based on different TaqMan™ probes and TaqMan™ MGB™ probes, in cocoa beans. Triplicate samples of a commercial cocoa bean were analysed using three different methods with three different probes and their respective TaqMan™ probes. The order of sensitivity for both toxins, based on Cq values, using the TaqMan™ MGB™ Probe was 18.64 for Ochratoxin A and 11.71 for citrinin. The overall data obtained using different probes showed a good correlation with all three methods giving an average standard deviation of 0.32. The sensitivity of the three methods was all above 88.1%, the quantification factor was always lower than 1.5 and the precision of the three methods was all below 5.2%. The ability to detect target toxins in cocoa samples using the currently reported TaqMan™ MGB™ probes showed satisfactory results and could be applied to the field after further validation studies.Czarności

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A study was carried out to compare the analytical performance of three different real-time PCR methods for the simultaneous detection of Ochratoxin A and citrinin, based on different TaqMan™ probes and TaqMan™ MGB™ probes, in cocoa beans. Triplicate samples of a commercial cocoa bean were analysed using three different methods with three different probes and their respective TaqMan™ probes. The order of sensitivity for both toxins, based on Cq values, using the TaqMan™ MGB™ Probe was 18.64 for Ochratoxin A and 11.71 for citrinin. The overall data obtained using different probes showed a good correlation with all three methods giving an average standard deviation of 0.32. The sensitivity of the three methods was all above 88.1%, the quantification factor was always lower than 1.5 and the precision of the three methods was all below 5.2%. The ability to detect target toxins in cocoa samples using the currently reported TaqMan™ MGB™ probes showed satisfactory results and could be applied to the field after further validation studies.Czarności

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