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Hepatocyte growth factor (HGF) is a pleiotropic cytokine which is essential for liver regeneration. Gene therapy has been considered to be an alternative treatment for acute liver failure; however, the application of adenoviral vector is hampered in clinical practice by its size and immunogenicity. To circumvent these problems, we employed small recombinant adenoviruses (RAds) as a possible substitute. First, we examined the ability of the adenovirus-expressing rat HGF (RAd-HGF) to up-regulate HGF mRNA and protein production in rat hepatocytes. Subsequently, we evaluated the therapeutic effect of RAd-HGF on an experimental model of acute liver failure induced by 3-acetyl-4-pyridinio-1-methylquinazoline (APAP). Expression of the RAd-HGF was confirmed in vitro using fluorescence microscopy, and its functionality was verified by the fact that transduced hepatocytes displayed biological activity by an increase of cell viability and a decrease of cell apoptosis induced by APAP. Subsequently, the therapeutic efficacy of RAd-HGF was examined in rats. Intravenous injection of RAd-HGF significantly reduced the serum activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and improved histological analysis. These results suggested that the RAd-HGF is able to successfully up-regulate the production of HGF and suppress the deleterious effects of APAP on hepatocytes in vivo.Acute folate deficiency induced by consumption of melinjo fruit, a rich source of polyphenols.
Studies conducted in New Zealand have shown that consuming the local melinjo fruit (Eureiopsis procera Benth.) could be a risk factor for folate deficiency. The aim of the current investigation was to examine the clinical and biochemical characteristics of folate deficiency during consumption of a local melinjo fruit diet. Clinical and biochemical characteristics were evaluated in five healthy young adults before and during
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