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High-risk neuroblastoma (NB) is associated with a poor prognosis, primarily due to metastatic dissemination of the disease. Here, we analyzed the expression of neuropilin-1 (NRP1) and NRP2 in three human NB cell lines (RD, IMR32 and Kelly), two isolated cell clusters of mouse NB primary tumors (CL1 and CL4), and 43 NB patients who had died after diagnosis. Our data showed: (i) NB cells express NRP1 and NRP2 mRNA and protein; (ii) in addition to NRP1, NRP2 is expressed in mouse NB tumors; and (iii) NRP1 and NRP2 were specifically expressed in NB tumor cell clusters and in tumor cells from 32 out of 43 primary NB tumors. More importantly, our data show that knockdown of NRP1/2 expression in the RD NB cell line reduced cell invasiveness and tumor cell clusters formation, whereas overexpression increased invasiveness and tumor cell clusters formation. Moreover, silencing of NRP1/2 expression also reduced metastasis of RD NB cells. Taken together, our data indicate that NRP1 and NRP2 function as tumor-intrinsic regulators of high-risk NB invasiveness and metastasis.Q:
Session states in Parley to control the states of the media streams
Basically I have a Speech stream and a Music stream.
I need to control the states of the music stream based on the speech state which is in the session scope from my application
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