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In conclusion, our study showed that dysregulated miR-34b/c expression may serve as a potential target to inhibit the growth and metastasis of NPC.
MATERIALS AND METHODS {#s4}
=====================
Ethics statement {#s4_1}
—————-
The study was approved by the Institutional Research and Ethics Committee of Tongji Medical College, Huazhong University of Science and Technology, the First Affiliated Hospital of Wuhan University and Tongji Hospital. Written informed consent was obtained from each individual patient prior to the study.
Patients and samples {#s4_2}
——————–
All NPC patient data were obtained from a prospectively maintained cohort of patients and followed-up at the Cancer Center of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology and Huazhong University of Science and Technology. The histological diagnosis and TNM staging for all patients was determined according to the World Health Organization (WHO) criteria and the International Union Against Cancer TNM Classification System.
In addition, forty-five nasopharyngeal biopsy specimens were obtained from untreated patients with newly diagnosed primary NPC and biopsy specimens were obtained from nasopharyngeal ulcerative tumors adjacent to NPC during surgery without neoadjuvant chemotherapy or radiotherapy before beginning treatment. Fifteen nasopharyngeal biopsy specimens were obtained from nasopharyngeal tumor patients with recurrent NPC who had received radiotherapy and three nasopharyngeal biopsy specimens were obtained from
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